Background: Several studies have suggested that knowledge, attitudes and determinants concerning ORGAN DONATION are influenced by many factors including gender, educational level, occupation, sociodemographic status, income level, culture and religion.Objective: To highlight the awareness of cardiopulmonary and brain death (CD and BD) among the physicians and their belief about the ORGAN DONATION.Methods: In a cross-sectional study, 15% of 1700 physicians working under the auspices of Ministry of Health in Makkah region, were selected randomly from two hospitals of Makkah city, i.e., Alnoor Specialist Hospital and King Abdalaziz Hospital. A self-administered questionnaire with dichotomous answers was distributed to them.Results: Out of 185 respondents, 174 (94.1%) identified the right definition of BD and CD and 155 (83.3%) agreed ORGAN DONATION. The difference among physicians to differentiate CD from BD was not significant (p=0.2).Conclusion: Physicians had enough knowledge to differentiate CD from BD; most of them are highly positive regarding the concept of ORGAN DONATION.